Affinity Ligands

Affinity ligands that show specific interaction with biomolecules can be attached to supports for affinity purification technologies, such as ligand affinity chromatography, membrane separation and aqueous two phase extraction. Efficient discrimination between the compound of interest and related impurities as well as finding a suitable linker strategy are the major tasks of affinity ligand development.

DESIGN AND SYNTHESIS OF AFFINITY LIGANDS

Syncom has broad experience in the design and custom synthesis of affinity ligands. These vary from simple weak anion or cation exchange ligands to more advanced ligands that for example show real affinity for immunoglobulins. Materials synthesized by Syncom have proved to be very effective for separation and purification of monoclonal antibodies from fermentation broths on laboratory, as well as pilot plant scale.).

LINKER STRATEGIES

In case of ligand affinity chromatography and membrane separation, ligand immobilization on the outer surface of a support is achieved by a linker, which might be a short linear molecule or branched polymer. For the case of aqueous two phase extraction, the ligand has to be attached to a soluble compound enabling the phase transfer of the target molecule. Depending on the functional groups on the support different linker strategies can be used to immobilize affinity ligands to the supports. Several linkers are available or can be tailor made by Syncom. Strategies vary from click chemistry to simple homo bifunctional or hetero bifunctional linkers that can react with the functional groups on the supports.

Syncom can deliver several affinity materials from stock. Syncom offers synthetic support for design and development of new affinity ligands, linker strategies and immobilization chemistry used in affinity purification technologies.